{"id":"hum0036","version":1,"url":"https://humandbs.dbcls.jp/research/hum0036/v1","datePublished":"2015-08-01","versions":[{"version":1,"datePublished":"2015-08-01"}],"title":{"ja":"全エクソームキャプチャプラットフォーム間の性能比較","en":"Performance Comparison of Four Commercial Human Whole-Exome Capture Platforms"},"summary":{"aims":{"ja":"市販されている4種類の全エクソームキャプチャープラットフォーム間の性能比較","en":"Performance comparison of four commercial human whole-exome capture platforms."},"methods":{"ja":"各エクソンキャプチャープラットフォーム (Roche/NimbleGen [SeqCap EZ Human Exome Library v3.0]、Illumina [Nextera Rapid Capture Exome（v1.2）]、Agilent [SureSelect XT Human All Exon v5、SureSelect QXT Human All Exon v5])ごとにシークエンスライブラリを作成し、Illumina社のHiSeq 2500により全エクソームシークエンシングを実施","en":"Each exome captured sequencing library was produced from one of four different technologies: Roche/NimbleGen's SeqCap EZ Human Exome Library v3.0, Illumina's Nextera Rapid Capture Exome (v1.2), Agilent's SureSelect XT Human All Exon v5 and Agilent's SureSelect QXT. The captured DNA was sequenced using the Illumina HiSeq 2500 platform with paired-end reads of 161 bp for insert libraries according to the manufacturer's instructions."},"targets":{"ja":"コリエル医学研究所より購入したHapMap-JPT検体由来のセルライン: 2名","en":"EBV-transformed lymphoblastoid cell lines (LCL) derived from two HapMap-JPT samples obtained from Coriell."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"キャプチャ方法の性能比較","en":"Capture platform comparison"},"targets":{"ja":"HapMap-JPT：2検体\n（日本人）","en":"2 HapMap-JPT samples\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"このバージョンでは、コリエル医学研究所より購入したHapMap-JPT検体由来のセルライン2検体を対象とし、Roche/NimbleGen [SeqCap EZ Human Exome Library v3.0]、Illumina [Nextera Rapid Capture Exome（v1.2）]、Agilent [SureSelect XT Human All Exon v5、SureSelect QXT Human All Exon v5] の4種類の各エクソンキャプチャプラットフォームをそれぞれ用いてエクソン領域を絞り込み、HiSeq 2500（Illumina）により全エクソームシーケンシングを実施した結果をfastq形式にて提供する（Paired-end: 161 bp x 2）。","en":"gDNAs extracted from EBV-transformed lymphoblastoid cell lines (LCL) derived from two HapMap-JPT samples obtained from Coriell were used for the whole exome sequencing (fastq files). DNA fragments containing whole coding exons were concentrated using SeqCap EZ Human Exome Library v3.0 (Roche/NimbleGen), Illumina [Nextera Rapid Capture Exome v1.2 (Illumina), SureSelect XT Human All Exon v5 (Agilent) or SureSelect QXT Human All Exon v5 (Agilent) and multiplex sequence was performed with the Illumina HiSeq 2500 sequencer (161 bp x 2, Paired-end)."},"dataProviders":[{"name":{"ja":"久保 充明","en":"Michiaki Kubo"},"organization":{"name":{"ja":"理化学研究所統合生命医科学研究センター","en":"RIKEN Center for Integrative Medical Science"}}}],"researchProjects":[{"name":{"ja":"疾患多様性医科学研究部門","en":"Core for Genomic Medicine"},"url":{"ja":null,"en":null}}],"grants":[],"relatedPublications":[{"title":"Performance comparison of four commercial human whole-exome capture platforms.","doi":"https://doi.org/10.1038/srep12742","datasets":["DRA003736"]}],"datasets":["DRA003736"],"controlledAccessUsers":[]}