{"id":"hum0034","version":1,"url":"https://humandbs.dbcls.jp/research/hum0034/v1","datePublished":"2015-10-01","versions":[{"version":1,"datePublished":"2015-10-01"}],"title":{"ja":"小細胞肺癌の網羅的ゲノム解析による新たな治療標的の探索を目指した研究","en":"Identification of novel therapeutic targets of small cell lung cancer by comprehensive genomic analyses"},"summary":{"aims":{"ja":"難治療性の小細胞肺がん（small cell lung cancer: SCLC）を対象として網羅的ゲノム解析を実施し、新たな治療標的を発見することにより新しい分子標的治療法の開発に結びつけること","en":"Identifying new therapeutic targets of small cell lung cancer (SCLC), genome-wide mutation analysis has been performed."},"methods":{"ja":"ホルマリン固定ないしメタノール固定された組織より抽出したゲノムDNA断片から SureSelect Human All Exon V4+UTRs Kit（Agilent Technologies）を用いて全エクソンを含む71Mb領域をキャプチャし、Illumina HiSeq 2000により100-bpペアエンドシークエンスを行った。","en":"Genomic DNA was extracted from formalin-fixed or methanol-fixed tissue samples. 71 Mb of DNA fragments containing whole coding exons were concentrated using SureSelect Human All Exon V4+UTRs Kit (Agilent Technologies) followed by 100-bp paired end sequencing by HiSeq 2000 (Illumina)."},"targets":{"ja":"1992-2012年に国立がん研究センター東病院 呼吸器内科の肺がんデータベースに登録された小細胞肺がん約1042例のうち、手術・生検が施行され、小細胞肺がんの病理診断が確定し、全エクソンシークエンスに必要なゲノムDNAが抽出可能だった51例","en":"51 of 1042 cases of pathologically diagnosed small seen lung cancer that were registered to National Cancer Hospital East Lung Cancer Database in 1992-2012, and which surgically resected or biopsy samples were suitable for DNA extraction for further analyses."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"SCLC：51症例\n（日本人）","en":"51 SCLCs\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"小細胞肺がんのホルマリン固定ないしメタノール固定された組織より抽出したゲノムDNAを用いたExome解析の結果をfastqファイルにて提供する。SureSelect Human All Exon V4+UTRs Kit（Agilent社）を用いて全エクソンを含む71Mb領域に絞り込み、Illumina社のHiSeq 2000による100-bpペアエンドシークエンスを行った。","en":"DNAs extracted formalin-fixed or methanol-fixed tissue samples of small seen lung cancer were used for the whole exome sequencing (fastq files). 71 Mb of DNA fragments containing whole coding exons were concentrated using SureSelect Human All Exon V4+UTRs Kit (Agilent Technologies) followed by 100-bp paired end sequencing by HiSeq 2000 (Illumina)."},"dataProviders":[{"name":{"ja":"後藤 功一","en":"Koichi Goto"},"organization":{"name":{"ja":"国立がん研究センター 東病院呼吸器内科","en":"Department of Thoracic Oncology, National Cancer Center Hospital East"}}}],"researchProjects":[{"name":{"ja":"難治性の小細胞肺がんを対象とした網羅的ゲノム解析による新しい分子標的治療法の開発","en":"Development of molecular targeted therapy for small cell lung cancer by comprehensive genome analysis"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"小細胞肺癌のゲノムワイド網羅的解析による発生分子基盤の解明と治療標的の探索","en":"Exploring therapeutic targets and investigating molecular carcinogenesis of small cell lung cancer by comprehensive genome analysis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["24300346"]},{"title":{"ja":"大細胞神経内分泌肺癌の標的遺伝子解析による新しい治療法の探索","en":"Development of molecular targeted therapy for large cell neuroendocrine carcinoma by targeting genome sequencing"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["26870876"]},{"title":{"ja":"がん研究分野における生物統計学・情報学研究基盤の構築に関する研究","en":"Development of the biostatistics and bioinformatics platforms for cancer research"},"agency":{"ja":"国立がん研究センターがん研究開発費","en":"National Cancer Center Research and Development Fund"},"grantIds":["23-A-8"]},{"title":{"ja":"がん治療の早期開発試験およびその研究体制確立に関する研究","en":"Development of the platforms for early phase clinical trials"},"agency":{"ja":"国立がん研究センターがん研究開発費","en":"National Cancer Center Research and Development Fund"},"grantIds":["23-A-15"]},{"title":{"ja":"小細胞肺がんの全ゲノム網羅的解析による治療標的の探索","en":"Development of molecular targeted therapy for small cell lung cancer by comprehensive genome analysis"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん研究シーズ戦略的育成プログラム（P-DIRECT）","en":"Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP15cm0106063"]}],"relatedPublications":[{"title":"Therapeutic priority of the PI3K/AKT/mTOR pathway in small cell lung cancers as revealed by a comprehensive genomic analysis.","doi":"https://doi.org/10.1097/JTO.0000000000000250","datasets":["JGAD000037"]}],"datasets":["JGAD000037"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Anton Berns"},"affiliation":{"en":"Division of Molecular Genetics, The Netherlands Cancer Institute"},"country":{"ja":"オランダ","en":"Netherlands"},"researchTitle":{"ja":"Integrative analysis of small cell lung cancer","en":"Integrative analysis of small cell lung cancer"},"periodStart":"2016-03-30","periodEnd":"2017-09-12","datasets":["JGAD000037"]},{"principalInvestigator":{"ja":"中岡 博史","en":"Hirofumi Nakaoka"},"affiliation":{"ja":"腫瘍ゲノム研究部, 公益財団法人佐々木研究所","en":"Department of Cancer Genome Research, Sasaki Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"肺がん遺伝子解析研究","en":"Genetic analysis of lung cancer"},"periodStart":"2022-08-17","periodEnd":"2026-07-23","datasets":["JGAD000037"]}]}