{"id":"hum0033","version":1,"url":"https://humandbs.dbcls.jp/research/hum0033/v1","datePublished":"2015-04-28","versions":[{"version":1,"datePublished":"2015-04-28"}],"title":{"ja":"ヒト唾液由来エキソソームの機能解析に関する研究","en":"Functional analysis of two types of exosome in human whole saliva"},"summary":{"aims":{"ja":"ヒト唾液には直径が30～100nmの膜小胞であるエキソソームIとエキソソームIIが存在している。本研究では、エキソソームI、エキソソームIIおよび全唾液に含まれるlong RNA（protein-coding RNA and long non-coding RNA）の量の比較を実施することを目的としている。","en":"Human whole saliva (WS) contains two types of exosomes (exosome I and exosome II) that are small membrane vesicle (30-100 nm in diameter). To clarify the host defense functions of exosomes, we investigated long RNA components (protein-coding RNAs and long non-coding RNAs) of exosome I, exosome II, and WS by next generation sequencing."},"methods":{"ja":"吐出法を用いて採取した唾液からエキソソームIとエキソソームIIを純化し、RNAを抽出した。Short non-coding RNAを除いたものをライブラリとし、次世代シークエンサーを用いてRNA量を検討した。","en":"Human whole saliva was collected by discharge. Long RNA components (protein-coding RNA and long non-coding RNA) of exosome I, exosome II, and WS were analyzed by next generation sequencing."},"targets":{"ja":"日本人成人健常者1名の唾液から純化したエキソソームI、エキソソームIIおよび全唾液を使用した。","en":"Human whole saliva was collected from one Japanese healthy female volunteer with written informed consent."},"url":{"ja":[{"url":"https://pharm.thu.ac.jp/research/unit/makukinou.html","text":"https://pharm.thu.ac.jp/research/unit/makukinou.html"}],"en":[{"url":"https://pharm.thu.ac.jp/research/unit/makukinou.html","text":"https://pharm.thu.ac.jp/research/unit/makukinou.html"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"健常者：1名（3検体）\n（日本人）","en":"1 Participant\n(3 Samples)\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"このバージョンでは、日本人成人女性（健常人）の全唾液、唾液から純化したエキソソームIおよびエキソソームIIから抽出したRNAの内、short non-coding RNAを除いたものをライブラリとし、Genome Analyzer IIx（Illumina）にて配列を読んでいる（Single-end: 36-51 bp）。","en":"In this version, total RNAs were extracted from human whole saliva (WS) and two types of salivary exosomes (exosome I and exosome II) purified from WS of a single healthy female Japanese (38 years old) and short RNA fraction was removed from total RNAs. cDNA library was constructed from the resulting RNAs. The cDNA was sequenced for 51 cycles, single-end, on the Illumina Genome Analyzer IIx. The primer/adapter sequences of the read sequences were removed and the trimmed reads with lengths shorter than 36 bases were discarded."},"dataProviders":[{"name":{"ja":"矢ノ下 良平","en":"Ryohei Yanoshita"},"organization":{"name":{"ja":"帝京平成大学・薬学部・薬学科","en":"Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Teikyo Heisei University"}}}],"researchProjects":[{"name":{"ja":"膜機能研究ユニット","en":"Membrane Function Research Unit"},"url":{"ja":[{"url":"https://pharm.thu.ac.jp/research/unit/makukinou.html","text":"https://pharm.thu.ac.jp/research/unit/makukinou.html"}],"en":null}}],"grants":[{"title":{"ja":"アミノペプチダーゼ内包エキソソームによる免疫活性化カスケードの分子機構とその制御","en":"Molecular mechanisms and regulations of immune activation cascades by exosomes expressing aminopeptidases."},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["25460172"]},{"title":{"ja":"医薬品開発に応用できる生体成分由来ナノキャリアの開発","en":"Development of a nanocarrier derived from biogenic substances for application to drug development."},"agency":{"ja":"科学技術振興機構 「産学共同シーズイノベーション化事業・顕在化ステージ」","en":"Japan Science and Technology Agency (JST) Industry-academia Joint Seeds Innovation Project"},"grantIds":null}],"relatedPublications":[{"title":"Small RNA transcriptomes of two types of exosomes in human whole saliva determined by next generation sequencing.","doi":"https://doi.org/10.1248/bpb.b12-00607","datasets":["DRA003516"]}],"datasets":["DRA003516"],"controlledAccessUsers":[]}