{"id":"hum0019","version":1,"url":"https://humandbs.dbcls.jp/research/hum0019/v1","datePublished":"2015-04-01","versions":[{"version":1,"datePublished":"2015-04-01"}],"title":{"ja":"消化器癌の発癌や進展に深く関与しうる遺伝子、並びに非コードRNAの量的・質的な異常の探索","en":"Systematic identification of long non-coding RNAs potentially involved in gastrointestinal cancer"},"summary":{"aims":{"ja":"消化器がんの発生・進展に重要な役割を果たす長鎖ncRNAの同定","en":"To identify novel long non-coding RNAs critically involved in gastrointestinal cancer."},"methods":{"ja":"Illumina HiSeq 2500を使用したRNA-seq解析","en":"Exome and RNA-seq experiments were performed to investigate quantitative and qualitative abnormalities of long non-coding RNAs in gastrointestinal cancer (Illumina HiSeq 2500)"},"targets":{"ja":"消化器がん17症例（腫瘍組織/非腫瘍組織、計32検体）","en":"Genomic DNA and polyA RNA were extracted from specimens in 17 gastrointestinal cancer patients. Cancer and non-cancerous tissues were used: total 32 samples)"},"url":{"ja":[{"url":"https://web.sapmed.ac.jp/biochem2/","text":"https://web.sapmed.ac.jp/biochem2/"}],"en":[{"url":"https://web.sapmed.ac.jp/biochem2/","text":"https://web.sapmed.ac.jp/biochem2/"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling, tumor vs. matched-normal"},"targets":{"ja":"消化器癌：17症例（32検体）\n（日本人）","en":"17 GICs\n(32 Samples)\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"このバージョンでは、日本人消化器癌患者17症例の消化器がん切片から腫瘍組織と非腫瘍組織を取り分け、polyA RNAを抽出してRNA-seqを実施した。Agilent SureSelect XT RNA Target Enrichment for Illumina Multiplexed Sequencingを用い、HiSeq 2500にて解読した結果をfastqファイルにて提供する（平均長100塩基 x 2）。","en":"RNA-seq was performed by using of polyA RNAs extracted from 17 gastrointestinal cancer and non-cancerous tissues (Illumina HiSeq 2500, 100bp x 2, paired-end)."},"dataProviders":[{"name":{"ja":"篠村 恭久","en":"Yasuhisa Shinomura"},"organization":{"name":{"ja":"札幌医科大学 消化器・免疫・リウマチ内科学講座","en":"Department of Gastroenterology, Rheumatology and Clinical Immunology, Sapporo Medical University"}}}],"researchProjects":[{"name":{"ja":"札幌医科大学・分子生物学講座との共同研究","en":"Collaborate with Department of Molecular Biology, Sapporo Medical University"},"url":{"ja":[{"url":"https://web.sapmed.ac.jp/biochem2/","text":"https://web.sapmed.ac.jp/biochem2/"}],"en":[{"url":"https://web.sapmed.ac.jp/biochem2/english","text":"https://web.sapmed.ac.jp/biochem2/english"}]}}],"grants":[{"title":{"ja":"大腸癌の発癌や進展に深く関与しうる長鎖ncRNAの量的・質的な異常の探索","en":"Systematic investigation of quantitative and qualitative abnormalities of long non-coding RNAs critically involved in colorectal cancer"},"agency":{"ja":"科学研究費助成事業 若手研究（A）","en":"KAKENHI Grant-in-Aid for Scientific Research (A)"},"grantIds":["24680092"]}],"relatedPublications":[],"datasets":["JGAD000011"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000011"]}]}