{"id":"hum0016","version":1,"url":"https://humandbs.dbcls.jp/research/hum0016/v1","datePublished":"2016-09-30","versions":[{"version":1,"datePublished":"2016-09-30"}],"title":{"ja":"精巣に発現するタンパク質群のヒト精子細胞における発現及び染色体局在解析","en":"Epigenome analysis of proteins expressed in human testis"},"summary":{"aims":{"ja":"精巣特異的クロマチン関連因子の精子細胞における発現を解析することで、精子細胞のクロマチン形成のメカニズムを解明する","en":"To elucidate the mechanism of chromatin formation in human sperm cells."},"methods":{"ja":"精子細胞において発現が認められた因子に関して、調整した精巣組織細胞のクロマチンを用いたChIP-seq解析（ヒストン抗体H3.5およびH3.3を使用）、および、対応検体のRNA-seq解析","en":"Analysis of ChIP-seq data of the testis-specific histone variant H3.5 (with H3.3 as control) and RNA-seq data in testis cells"},"targets":{"ja":"正常ヒト精巣組織細胞","en":"Normal testis cells"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nヒストン修飾","en":"Expression profiling, histone modification profiling"},"targets":{"ja":"健常者：1名（4検体）\n（日本人）","en":"1 Participant\n(4 Samples)\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq、ChIP-seq）","en":"NGS\n(ChIP-seq, RNA-seq)"}},"releaseNote":{"ja":"このバージョンでは、1名の正常ヒト精巣組織細胞を対象とし、ヒストン抗体H3.5およびH3.3を使用したIPによりライブラリを作成したChIP-seqおよび同検体のInput data、ならびにRNA-seq（Illumina HiSeq 1500）のfastqファイルを提供する（Single-end： 50 bp）。","en":"ChIP-seq data (IP with histone specific antibodies: H3.3 and H3.5), Input data, and RNA-seq data from one human adult testis cells are provided (Illumina HiSeq 1500, single-end, 50 bp)."},"dataProviders":[{"name":{"ja":"大川 恭行","en":"Yasuyuki Ohkawa"},"organization":{"name":{"ja":"九州大学 生体防御医学研究所 トランスクリプトミクス分野","en":"Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu university"}}}],"researchProjects":[],"grants":[{"title":{"ja":"動的クロマチン構造と機能（領域番号：3506） 細胞分化にともなうクロマチン変動メカニズムの解明","en":"Exploring chromatin dynamics in cell differentiation"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area: Dynamic chromatin structure and function)"},"grantIds":["25116010"]}],"relatedPublications":[{"title":"Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis","doi":"https://doi.org/10.1186/s13072-016-0051-y","datasets":["DRA002604"]}],"datasets":["DRA002604"],"controlledAccessUsers":[]}