{"id":"hum0011","version":1,"url":"https://humandbs.dbcls.jp/research/hum0011/v1","datePublished":"2015-10-07","versions":[{"version":1,"datePublished":"2015-10-07"}],"title":{"ja":"B型肝炎ウイルスのヒトゲノム内組み込みによるエピジェネティックな修飾","en":"Integrated hepatitis B virus is epigenomically regulated by the methylation status of the human genome"},"summary":{"aims":{"ja":"B型肝炎ウィルスのヒトゲノム内への組み込み解析研究","en":"To clarify integrated HBV sequences in the human genome."},"methods":{"ja":"Target enrichment systemによりHBVゲノム組み込み部位を含むヒトゲノムをキャプチャし、ライブラリとして用いた次世代シークエンス解析","en":"Target enrichment sysem-based next generation sequencer"},"targets":{"ja":"PLC/PRF/5細胞株1サンプル、HepG22215細胞株1サンプル、HBV感染ヒト肝がん 8症例","en":"PLC/PRF/5 cell, HepG22215 cell, and HB-HCC primary tissues"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"HBV組込部位\n配列決定","en":"Sequencing of HBV integration sites"},"targets":{"ja":"2検体（セルライン）\nHBV感染肝癌：8症例\n（日本人）","en":"2 Cell lines\n8 HB-HCC primary tissues\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"PLC/PRF/5細胞株1サンプル、HepG22215細胞株1サンプル、HBV感染ヒト肝がん8症例のHBVゲノム組み込み部位をライブラリとした次世代シークエンス解析（Roche GS FLX Titanium）。multi-FASTA形式、 Quality value、SFF形式（454 Reads）のファイルを提供します。","en":"Exome sequense analysis using nextgenaration sequencer (Roche GS FLX Titanium) about integrated HBV sequences from 1 PLC/PRF/5 cell, 1 HepG22215 cell, 8 HB-HCC primary tissues. Multi-FASTA format files, Quality value files, and SFF format files (454 Reads) were provided."},"dataProviders":[{"name":{"ja":"山本 博幸","en":"Hiroyuki Yamamoto"},"organization":{"name":{"ja":"聖マリアンナ医科大学 消化器・肝臓内科","en":"St. Marianna University School of Medicine, Division of Gastroenterology and Hepatology"}}}],"researchProjects":[],"grants":[{"title":{"ja":"B型肝炎の肝発癌におけるHBx遺伝子の役割の網羅的解析","en":"Sequence analysis at HBV integrants and flanking host genomes"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["23590964"]}],"relatedPublications":[{"title":"DNA methylation at hepatitis B viral integrants is associated with methylation at flanking human genomic sequences.","doi":"https://doi.org/10.1101/gr.175240.114","datasets":["JGAD000015"]}],"datasets":["JGAD000015"],"controlledAccessUsers":[]}