{"id":"hum0009","version":3,"url":"https://humandbs.dbcls.jp/research/hum0009/v3","datePublished":"2015-08-03","versions":[{"version":1,"datePublished":"2014-12-24"},{"version":2,"datePublished":"2015-01-07"},{"version":3,"datePublished":"2015-08-03"}],"title":{"ja":"ヒト初期発生にかかわる細胞のDNAメチル化解析基盤研究","en":"DNA Methylation Analysis of Early Human Embryos"},"summary":{"aims":{"ja":"ヒト初期発生過程におけるDNAメチル化ダイナミクスの解明","en":"To generate single-base resolution DNA methylome maps of human gametes and embryos and uncover DNA methylation dynamics during early human development."},"methods":{"ja":"post-bisulfite adaptor tagging（PBAT）法によりライブラリー調製をし、Illimina社 HiSeq 2000もしくは2500を使用したメチル化解析","en":"Whole genome bisulfite sequencing libraries were prepared using the post-bisulfite adaptor tagging (PBAT) method. PBAT libraries were sequenced on the HiSeq 2000 or 2500 platform (Illumina) with 100 bp single-end reads."},"targets":{"ja":"日本人正常卵子、精子、受精卵（着床前胚）、臍帯血から抽出したゲノムDNA","en":"Human oocytes, sperm, preimplantation embryos, and cord blood cells"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"メチル化シトシン同定","en":"Identification of methylated cytosines"},"targets":{"ja":"11検体\n（日本人）","en":"11 Libraries\n(Japanese)"},"typeOfData":{"ja":"NGS\n（PBAT-seq）","en":"NGS\n(PBAT-seq)"}},"releaseNote":{"ja":"このバージョンでは、制限公開データ（Type I）として当初公開していたJGAD000006のアクセス制限（制限公開[Type I]）を非制限公開データ（DRA003802）に変更し、公開を開始した。データのダウンロードはDRAから。","en":"The access level of JGAD000006 (Controlled-access [Type I]) was changed to \"Unrestricted-access\" (DRA003802). You can have the data through the DRA site."},"dataProviders":[{"name":{"ja":"有馬 隆博","en":"Takahiro Arima"},"organization":{"name":{"ja":"東北大学大学院 医学系研究科 環境遺伝医学総合研究センター 情報遺伝学分野","en":"Department of Informative Genetics, Environment and Genome Research Center,Tohoku University Graduate School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"生殖発生にかかわる細胞のエピゲノム解析基盤研究","en":"Epigenome analysis of cells in the placenta and endometrium forming the fetal-maternal interface"},"agency":{"ja":"日本医療研究開発機構 革新的先端研究開発支援事業（AMED-CREST）","en":"Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)"},"grantIds":["JP17gm0510011"]},{"title":{"ja":"ヒト生殖細胞のエピゲノムダイナミクスとその制御機構","en":"Epigenome Dynamics and Regulation in Germ Cells"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["26112502"]}],"relatedPublications":[{"title":"Genome-Wide Analysis of DNA Methylation Dynamics during Early Human Development","doi":"https://doi.org/10.1371/journal.pgen.1004868","datasets":["DRA003802","JGAD000006"]}],"datasets":["DRA003802","JGAD000006","NHA000004"],"controlledAccessUsers":[{"principalInvestigator":{"en":"David Monk"},"affiliation":{"en":"Bellvitge Institute for Biomedical Research (IDIBELL)"},"country":{"ja":"スペイン","en":"Spain"},"researchTitle":{"ja":"胎盤におけるメチル化領域の生殖系由来を追跡すること。ヒト単為生殖幹細胞の発生期に異常に消失した卵母細胞由来のメチル化を特定すること。","en":"To trace the germline origin of methylated regions in the placenta. To identify oocyte-derived methylation that is aberrantly lost during human parthenogenetic stem cell generation."},"periodStart":"2015-04-07","periodEnd":"2015-08-04","datasets":["JGAD000006"]},{"principalInvestigator":{"ja":"藤 英博","en":"Hidehiro Toh"},"affiliation":{"ja":"ゲノム機能制御学部門 エピゲノム制御学分野, 九州大学生体防御医学研究所","en":"Division of Epigenomics and Development, Medical Institute of Bioregulation Kyushu University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"生殖発生にかかわる細胞のエピゲノム解析基盤研究","en":"Epigenome dynamics and regulation in germ cells"},"periodStart":"2015-04-01","periodEnd":"2015-08-04","datasets":["JGAD000006"]},{"principalInvestigator":{"en":"David Skaar"},"affiliation":{"en":"Department of Biological Sciences, North Carolina State University"},"country":{"ja":"アメリカ合衆国 (ノースカロライナ州)","en":"North Carolina, United States"},"researchTitle":{"ja":"Epigenetics and Genetics Throughout the Lifespan","en":"Epigenetics and Genetics Throughout the Lifespan"},"periodStart":"2019-04-15","periodEnd":"2021-02-02","datasets":["JGAD000006"]}]}