{"id":"hum0007","version":1,"url":"https://humandbs.dbcls.jp/research/hum0007/v1","datePublished":"2015-02-12","versions":[{"version":1,"datePublished":"2015-02-12"}],"title":{"ja":"ヒトTh17細胞の活性化前後の遺伝子発現","en":"Gene Expression of Human Th17 Cells Before and After Activation"},"summary":{"aims":{"ja":"Th1細胞およびTh17細胞における遺伝子発現を解析することで、自己免疫疾患の原因解明や治療方法の確立を目指す","en":"To clarify the mechanism of auto-immune diseases and establish the treatment for auto-immune diseases, through analyzing the expression pattarns of Th1 cells and Th17 cells."},"methods":{"ja":"AppliedBioSystem SoLiDを使用したRNA-Seq解析","en":"RNA-Seq by using of AppliedBioSystem SoLiD system"},"targets":{"ja":"健常者1名の末梢血から単離したナイーブT細胞、Th1細胞、inactive-Th17、active-Th17","en":"Total RNAs were extracted from naïve T cells, CXCR3+Th1 cells, CD161+Th17 cells, and activated Th17 cells for one individual"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"健常者：1名\n（日本人）","en":"1 Participant\n(4 Samples)\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"このバージョンでは、日本人健常人1名からのナイーブT細胞、Th1細胞、活性化Th17細胞、非活性化Th17細胞から抽出したRNAを用いたRNA-seq解析の結果をcsfastaおよびqualフォーマットファイルにて提供する。AppliedBioSystems社のSOLiDキットを用い、AB5500 Genetic Analyzerにて解読した（平均長35塩基）。","en":"Total RNAs were extracted from naïve T cells, CXCR3+Th1 cells, CD161+Th17 cells, and activated Th17 cells of one individual, and the sequences were read by using of AB5500 Genetic Analyzer (35bp). The sequences are provided as csfasta or qual format."},"dataProviders":[{"name":{"ja":"巌本 三壽","en":"Sanju Iwamoto"},"organization":{"name":{"ja":"昭和大学医学部 生化学教室","en":"Department of Biochemistry, Showa University School of Medicine"}}}],"researchProjects":[{"name":{"ja":"ヒトTh17細胞サブセットに関する研究","en":"Research for human Th17 subset"},"url":{"ja":[{"url":"https://www.showa-u.ac.jp/education/med/major/biochem.html","text":"https://www.showa-u.ac.jp/education/med/major/biochem.html"}],"en":[{"url":"https://www.showa-u.ac.jp/education/med/major/biochem.html","text":"https://www.showa-u.ac.jp/education/med/major/biochem.html"}]}}],"grants":[],"relatedPublications":[{"title":"TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses.","doi":"https://doi.org/10.4049/jimmunol.179.3.1449","datasets":["JGAD000005"]}],"datasets":["JGAD000005"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000005"]}]}