{"id":"hum0003","version":1,"url":"https://humandbs.dbcls.jp/research/hum0003/v1","datePublished":"2013-07-01","versions":[{"version":1,"datePublished":"2013-07-01"}],"title":{"ja":"関節リウマチ患者及び健常人におけるHLA領域の塩基配列比較解析","en":"Comparative sequence analysis of the HLA region in patients with rheumatoid arthritis and healthy individuals"},"summary":{"aims":{"ja":"HLA遺伝子(HLA-A, -C, -B, -DRB1, -DQB1および-DPB1)におけるHLAアレル完全長塩基配列(約3kb〜14kb)を決定する","en":"To determine the full-length sequences (about 3 kb to 14 kb) of the HLA alleles of the HLA genes (HLA-A, -C, -B, -DRB1, -DQB1 and -DPB1)"},"methods":{"ja":"Long range PCRにより目的領域を増幅し、Illumina社のMiSeqにより塩基配列を解析する","en":"The target regions are amplified by long-range PCR and sequenced with MiSeq (Illumina)"},"targets":{"ja":"複数集団由来セルライン：33名","en":"Cell lines from multiple populations: 33 individuals"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"HLA領域\n配列決定","en":"HLA region sequencing"},"targets":{"ja":"33検体\n（細胞株）","en":"33 samples\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"このバージョンでは、6つのHLA遺伝子(HLA-A, -C, -B, DRB1, -DQB1, -DPB1)領域のLong-range PCRを行ない、Nextera DNA Preparation Kitを用いて断片化とアダプターの付加を行なった後、MiSeq (Illumina)にて配列を読んでいる（Paired-end: 2 x 250 bp）。","en":"In this version, the regions of 6 HLA genes (HLA-A, -C, -B, DRB1, -DQB1, -DPB1) were amplified by long-range PCR, fragmented and tagged with adapters using the Nextera DNA Preparation Kit, and sequenced with MiSeq (Illumina) (paired-end: 2 x 250 bp)."},"dataProviders":[{"name":{"ja":"井ノ上 逸朗","en":"Ituro Inoue"},"organization":{"name":{"ja":"国立遺伝学研究所 人類遺伝研究部門","en":"Division of Human Genetics, National Institute of Genetics"}}}],"researchProjects":[],"grants":[{"title":{"ja":"HLA進化と疾病 リシークエンシングによるHLAゲノム多様性解析","en":"HLA genome diversity analyses by resequencing"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["22133002"]},{"title":{"ja":"医学系研究奨励 (2011年度)"},"agency":{"ja":"公益財団法人 武田科学振興財団","en":"Takeda Science Foundation"},"grantIds":null},{"title":{"ja":"ゲノム科学の総合的推進に向けた大規模ゲノム情報生産・ 高度情報解析支援","en":"Platform of large scale and high quality genomics and bioinformatics: Towards the advancement of genome sciences in academia"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["221S0002"]}],"relatedPublications":[{"title":"Phase-defined complete sequencing of the HLA genes by next-generation sequencing.","doi":"https://doi.org/10.1186/1471-2164-14-355","datasets":["DRA000908"]}],"datasets":["DRA000908"],"controlledAccessUsers":[]}