{"id":"JGAD000816","research":"hum0302","url":"https://humandbs.dbcls.jp/dataset/JGAD000816","datePublished":"2024-03-15","dateModified":"2024-03-15","values":[{"key":"access-criteria","label":{"ja":"アクセス制限","en":"Access criteria"},"type":"vocabulary","terms":[{"code":"controlled-access-type-1","label":{"ja":"制限公開（Type I）","en":"Controlled-access (Type I)"}}]},{"key":"type-of-data","label":{"ja":"データの種類","en":"Type of data"},"type":"text","text":{"ja":"NGS（RNA-seq）","en":"NGS (RNA-seq)"}}],"experiments":[{"label":"RNA-seq","values":[{"key":"materials-and-participants","label":{"ja":"材料と対象者","en":"Materials and participants"},"type":"text","text":{"ja":"若年性ネフロン癆（NPH1）（ICD10：N19）患者由来iPS細胞から分化誘導させた腎臓オルガノイドサンプル：6検体\n若年性ネフロン癆（NPH1）患者由来iPS細胞に責任遺伝子NPHP1を過剰発現させてから分化誘導させた腎臓オルガノイドサンプル：6検体\n健常人由来iPS細胞から分化誘導させた腎臓オルガノイドサンプル：3検体\n健常人由来iPS細胞をゲノム編集し、NPHP1変異を導入した腎臓オルガノイドサンプル：3検体\n計18検体","en":"6 kidney organoid samples induced from juvenile nephron tabes (NPH1) patient-derived iPS cells (ICD10: N19)\n6 kidney organoid samples induced from juvenile nephron tabes (NPH1) patient-derived iPS cells overexpressing the responsible gene NPHP1\n3 kidney organoid samples induced to differentiate from healthy human iPS cells\n3 kidney organoid samples in which NPHP1 mutation was introduced by genome editing of healthy human iPS cells\n[Total: 18 samples]"}},{"key":"disease","label":{"ja":"疾患","en":"Disease"},"type":"disease","diseases":[{"terms":[{"code":"N19","label":{"ja":"詳細不明の腎不全","en":"Unspecified kidney failure"}}],"name":{"ja":"若年性ネフロン癆（NPH1）","en":"juvenile nephron tabes (NPH1)"}}]},{"key":"health-status","label":{"ja":"健康状態","en":"Health status"},"type":"vocabulary","terms":[{"code":"mixed","label":{"ja":"混在","en":"Mixed"}}]},{"key":"subject-count","label":{"ja":"対象者数","en":"Subject count"},"type":"number","numbers":[{"value":30,"unit":null,"high":null}]},{"key":"subject-count-type","label":{"ja":"対象者数の数え方","en":"Counted as"},"type":"vocabulary","terms":[{"code":"sample","label":{"ja":"検体数","en":"Sample"}}]},{"key":"sample-description","label":{"ja":"試料説明","en":"Sample description"},"type":"text","text":{"ja":"iPS細胞から分化誘導させた肝細胞および腎臓オルガノイドから抽出したRNA","en":"RNAs extracted from hepatocytes and kidney organoids differentiated from iPS cells"}},{"key":"tissue","label":{"ja":"組織","en":"Tissue"},"type":"vocabulary","terms":[{"code":"hepatocyte","label":{"en":"Hepatocyte"}},{"code":"kidney-organoid","label":{"en":"Kidney organoid"}}]},{"key":"is-tumor","label":{"ja":"腫瘍 / 非腫瘍","en":"Tumor / normal"},"type":"vocabulary","terms":[{"code":"normal","label":{"ja":"非腫瘍","en":"Normal"}}]},{"key":"cell-line","label":{"ja":"細胞株","en":"Cell line"},"type":"vocabulary","terms":[{"code":"ipsc","label":{"en":"iPSC"}}]},{"key":"sample-provider","label":{"ja":"試料の入手元","en":"Sample provider"},"type":"text","text":{"ja":null,"en":null}},{"key":"experimental-method","label":{"ja":"実験方法","en":"Experimental method"},"type":"vocabulary","terms":[{"code":"rna-seq","label":{"en":"RNA-seq"}}]},{"key":"targets","label":{"ja":"測定対象","en":"Target"},"type":"text","text":{"ja":null,"en":null}},{"key":"fragmentation","label":{"ja":"断片化","en":"Fragmentation"},"type":"text","text":{"ja":"GeneWiz社のプロトコルに準拠","en":"GeneWiz inc.'s protocol"}},{"key":"platform","label":{"ja":"プラットフォーム","en":"Platform"},"type":"vocabulary","terms":[{"code":"illumina-novaseq-6000","label":{"en":"Illumina NovaSeq 6000"}}]},{"key":"read-type","label":{"ja":"リードタイプ","en":"Read type"},"type":"vocabulary","terms":[{"code":"paired-end","label":{"ja":"ペアエンド","en":"Paired-end"}}]},{"key":"read-length","label":{"ja":"リード長","en":"Read length"},"type":"number","numbers":[{"value":150,"unit":"bp","high":null}]},{"key":"policies","label":{"ja":"利用ポリシー","en":"Data use policy"},"type":"vocabulary","terms":[{"code":"nbdc-data-sharing-policy-jgap000001","label":{"ja":"NBDC データ共有ポリシー (JGAP000001)","en":"NBDC data sharing policy (JGAP000001)"}}]}]}],"dataVolume":72927182517,"fileFormats":[{"code":"fastq","label":{"en":"FASTQ"}}]}