{"id":"JGAD000602","research":"hum0214","url":"https://humandbs.dbcls.jp/dataset/JGAD000602","datePublished":"2023-07-10","dateModified":"2023-07-10","values":[{"key":"access-criteria","label":{"ja":"アクセス制限","en":"Access criteria"},"type":"vocabulary","terms":[{"code":"controlled-access-type-1","label":{"ja":"制限公開（Type I）","en":"Controlled-access (Type I)"}}]},{"key":"type-of-data","label":{"ja":"データの種類","en":"Type of data"},"type":"text","text":{"ja":"RNA-seqによるB細胞免疫細胞サブセットのB細胞受容体レパトアデータ","en":"B cell receptor repertoire clonotype data from B cell RNA-seq"}}],"experiments":[{"label":{"ja":"RNA-seq + BCR-seq","en":"RNA-seq + BCR-seq"},"values":[{"key":"materials-and-participants","label":{"ja":"材料と対象者","en":"Materials and participants"},"type":"text","text":{"ja":"全身性エリテマトーデス（ICD10：M329）：136症例（159検体、23検体は経時サンプル）\n全身性強皮症（ICD10：M340）：90症例（90検体）\n筋炎（ICD10：M339、M332）：85症例（85検体）\n混合性結合組織病（ICD10：M351）：21症例（21検体）\nシェーグレン症候群（ICD10：M350）：18症例（18検体）\n関節リウマチ（ICD10：M0690）：27症例（27検体）\nベーチェット病（ICD10：M352）：23症例（23検体）\n成人スチル病（ICD10：M0610）：18症例（18検体）\nANCA関連血管炎（ICD10：M318）：24症例（24検体）\n高安動脈炎（ICD10：M314）：16症例（16検体）\n対照健常者：13名（13検体）","en":"Systemic Lupus Erythematosus (ICD10: M329): 136 cases (159 samples)\nSystemic Sclerosis (ICD10: M340): 90 cases (90 samples)\nMyositis (ICD10: M339, M332): 85 cases (85 samples)\nMixed Connective Tissue Disease (ICD10: M351): 21 cases (21 samples)\nSjögren's Syndrome (ICD10: M350): 18 cases (18 samples)\nRheumatoid Arthritis (ICD10: M0690): 27 cases (27 samples)\nBehçet's Disease (ICD10: M352): 23 cases (23 samples)\nAdult Onset Still's Disease (ICD10: M0610): 18 cases (18 samples)\nANCA-associated Vasculitis (ICD10: M318): 24 cases (24 samples)\nTakayasu's Arteritis (ICD10: M314): 16 cases (16 samples)\n13 healthy controls (13 samples)"}},{"key":"disease","label":{"ja":"疾患","en":"Disease"},"type":"disease","diseases":[{"terms":[{"code":"M329","label":{"ja":"全身性エリテマトーデス＜紅斑性狼瘡＞＜SLE＞，詳細不明","en":"Systemic lupus erythematosus, unspecified"}}],"name":{"ja":"全身性エリテマトーデス","en":"Systemic Lupus Erythematosus"}},{"terms":[{"code":"M340","label":{"ja":"全身性進行性硬化症","en":"Progressive systemic sclerosis"}}],"name":{"ja":"全身性強皮症","en":"Systemic Sclerosis"}},{"terms":[{"code":"M339","label":{"ja":"皮膚（多発性）筋炎，詳細不明","en":"Dermatopolymyositis, unspecified"}},{"code":"M332","label":{"ja":"多発性筋炎","en":"Polymyositis"}}],"name":{"ja":"筋炎","en":"Myositis"}},{"terms":[{"code":"M351","label":{"ja":"その他の重複症候群","en":"Other overlap syndromes"}}],"name":{"ja":"混合性結合組織病","en":"Mixed Connective Tissue Disease"}},{"terms":[{"code":"M350","label":{"ja":"乾燥症候群［シェーグレン＜Sjogren＞症候群］","en":"Sicca syndrome [Sjögren]"}}],"name":{"ja":"シェーグレン症候群","en":"Sjögren's Syndrome"}},{"terms":[{"code":"M069","label":{"ja":"関節リウマチ，詳細不明","en":"Rheumatoid arthritis, unspecified"}}],"name":{"ja":"関節リウマチ","en":"Rheumatoid Arthritis"}},{"terms":[{"code":"M352","label":{"ja":"ベーチェット＜Behcet＞病","en":"Behçet disease"}}],"name":{"ja":"ベーチェット病","en":"Behçet's Disease"}},{"terms":[{"code":"M061","label":{"ja":"成人発症スチル＜Still＞病","en":"Adult-onset Still disease"}}],"name":{"ja":"成人スチル病","en":"Adult Onset Still's Disease"}},{"terms":[{"code":"M318","label":{"ja":"その他の明示されたえ＜壊＞死性血管障害","en":"Other specified necrotizing vasculopathies"}}],"name":{"ja":"ANCA関連血管炎","en":"ANCA-associated Vasculitis"}},{"terms":[{"code":"M314","label":{"ja":"大動脈弓症候群［高安病］","en":"Aortic arch syndrome [Takayasu]"}}],"name":{"ja":"高安動脈炎","en":"Takayasu's Arteritis"}}]},{"key":"health-status","label":{"ja":"健康状態","en":"Health status"},"type":"vocabulary","terms":[{"code":"healthy","label":{"ja":"健常","en":"Healthy"}}]},{"key":"sample-description","label":{"ja":"試料説明","en":"Sample description"},"type":"text","text":{"ja":"末梢血B細胞5画分（Naive_B, USM_B, SM_B, DN_B, Plasmablast）より抽出したtotal RNA","en":"Total RNAs extracted from 5 B cell subsets (Naive_B, USM_B, SM_B, DN_B, Plasmablast)"}},{"key":"tissue","label":{"ja":"組織","en":"Tissue"},"type":"vocabulary","terms":[{"code":"b-cell","label":{"ja":"B 細胞","en":"B cell"}},{"code":"peripheral-blood","label":{"ja":"末梢血","en":"Peripheral blood"}}]},{"key":"sample-provider","label":{"ja":"試料の入手元","en":"Sample provider"},"type":"text","text":{"ja":null,"en":null}},{"key":"experimental-method","label":{"ja":"実験方法","en":"Experimental method"},"type":"vocabulary","terms":[{"code":"rna-seq","label":{"en":"RNA-seq"}},{"code":"bcr-seq","label":{"en":"BCR-seq"}}]},{"key":"targets","label":{"ja":"測定対象","en":"Target"},"type":"text","text":{"ja":null,"en":null}},{"key":"reagents","label":{"ja":"試薬キット","en":"Reagent kit"},"type":"vocabulary","terms":[{"code":"smart-seq-v4-ultra-low-input-rna-kit","label":{"en":"SMART-Seq v4 Ultra Low Input RNA Kit"}}]},{"key":"fragmentation","label":{"ja":"断片化","en":"Fragmentation"},"type":"text","text":{"ja":"SMART-Seq v4 Ultra Low Input RNA Kit","en":"SMART-Seq v4 Ultra Low Input RNA Kit"}},{"key":"platform","label":{"ja":"プラットフォーム","en":"Platform"},"type":"vocabulary","terms":[{"code":"illumina-hiseq-2500","label":{"en":"Illumina HiSeq 2500"}}]},{"key":"read-type","label":{"ja":"リードタイプ","en":"Read type"},"type":"vocabulary","terms":[{"code":"paired-end","label":{"ja":"ペアエンド","en":"Paired-end"}}]},{"key":"read-length","label":{"ja":"リード長","en":"Read length"},"type":"number","numbers":[{"value":100,"unit":"bp","high":null}]},{"key":"mapping","label":{"ja":"マッピング","en":"Mapping"},"type":"text","text":{"ja":"MiXCR","en":"MiXCR"}},{"key":"filtering","label":{"ja":"QC・フィルタリング","en":"QC and filtering"},"type":"text","text":{"ja":"アダプター配列及び3'の低クオリティー塩基をトリムした（Phred quality score < 20）。50塩基未満のリード、及び低クオリティーの塩基を多く含むリード（20%を超える塩基で、Phred quality score < 20）を除去した。 ユニークなCDR-H3配列を 500より多く有するサンプルのみ解析に使用した。","en":"The adaptor sequences and 3' low quality bases (Phred quality score < 20) were trimmed. Short reads (< 50bp) and reads containing many low quality bases (Phred quality score < 20 in > 20% of the bases) were removed.Samples with more than 500 unique CDR-H3 sequences were used for the analysis."}},{"key":"analysis-methods","label":{"ja":"解析方法","en":"Analysis method"},"type":"text","text":{"ja":"MiXCR","en":"MiXCR"}},{"key":"processed-data-type","label":{"ja":"加工データの種類","en":"Processed data type"},"type":"vocabulary","terms":[{"code":"b-cell-receptor-repertoire-clonotype-data","label":{"en":"B cell receptor repertoire clonotype data"}}]},{"key":"policies","label":{"ja":"利用ポリシー","en":"Data use policy"},"type":"vocabulary","terms":[{"code":"nbdc-data-sharing-policy-jgap000001","label":{"ja":"NBDC データ共有ポリシー (JGAP000001)","en":"NBDC data sharing policy (JGAP000001)"}}]}]}],"dataVolume":12506346191,"fileFormats":[{"code":"txt","label":{"en":"TXT"}}]}