{"id":"JGAD000497","research":"hum0302","url":"https://humandbs.dbcls.jp/dataset/JGAD000497","datePublished":"2021-11-10","dateModified":"2021-11-10","values":[{"key":"access-criteria","label":{"ja":"アクセス制限","en":"Access criteria"},"type":"vocabulary","terms":[{"code":"controlled-access-type-1","label":{"ja":"制限公開（Type I）","en":"Controlled-access (Type I)"}}]},{"key":"type-of-data","label":{"ja":"データの種類","en":"Type of data"},"type":"text","text":{"ja":"NGS（RNA-seq）","en":"NGS (RNA-seq)"}}],"experiments":[{"label":"RNA-seq","values":[{"key":"materials-and-participants","label":{"ja":"材料と対象者","en":"Materials and participants"},"type":"text","text":{"ja":"ウィルソン病（ICD10：E830）患者由来iPS細胞から分化誘導させた肝細胞サンプル：5検体\nウイルソン病患者由来iPS細胞をゲノム編集し、ATP7B変異を修復した肝細胞サンプル：1検体\n健常人由来iPS細胞から分化誘導させた肝細胞サンプル：5検体\n健常人由来iPS細胞をゲノム編集し、ATP7B変異を導入した肝細胞サンプル：1検体\n計12検体","en":"Five samples of hepatocytes differentiated from iPS cells derived from patients with Wilson's disease (ICD10: E830)\nOne hepatocyte sample from a patient with Wilson's disease in which the ATP7B mutation was corrected by genome editing of iPS cells\nFive samples of hepatocytes differentiated from healthy human-derived iPS cells\nOne hepatocyte sample in which the ATP7B mutation was introduced by genome editing of healthy donor-derived iPS cells\n[Total: 12 samples]"}},{"key":"disease","label":{"ja":"疾患","en":"Disease"},"type":"disease","diseases":[{"terms":[{"code":"E830","label":{"ja":"銅代謝障害","en":"Disorders of copper metabolism"}}],"name":{"ja":"ウィルソン病","en":"Wilson's disease"}}]},{"key":"health-status","label":{"ja":"健康状態","en":"Health status"},"type":"vocabulary","terms":[{"code":"mixed","label":{"ja":"混在","en":"Mixed"}}]},{"key":"subject-count","label":{"ja":"対象者数","en":"Subject count"},"type":"number","numbers":[{"value":30,"unit":null,"high":null}]},{"key":"subject-count-type","label":{"ja":"対象者数の数え方","en":"Counted as"},"type":"vocabulary","terms":[{"code":"sample","label":{"ja":"検体数","en":"Sample"}}]},{"key":"sample-description","label":{"ja":"試料説明","en":"Sample description"},"type":"text","text":{"ja":"iPS細胞から分化誘導させた肝細胞および腎臓オルガノイドから抽出したRNA","en":"RNAs extracted from hepatocytes and kidney organoids differentiated from iPS cells"}},{"key":"tissue","label":{"ja":"組織","en":"Tissue"},"type":"vocabulary","terms":[{"code":"hepatocyte","label":{"en":"Hepatocyte"}},{"code":"kidney-organoid","label":{"en":"Kidney organoid"}}]},{"key":"is-tumor","label":{"ja":"腫瘍 / 非腫瘍","en":"Tumor / normal"},"type":"vocabulary","terms":[{"code":"normal","label":{"ja":"非腫瘍","en":"Normal"}}]},{"key":"sample-provider","label":{"ja":"試料の入手元","en":"Sample provider"},"type":"text","text":{"ja":null,"en":null}},{"key":"experimental-method","label":{"ja":"実験方法","en":"Experimental method"},"type":"vocabulary","terms":[{"code":"rna-seq","label":{"en":"RNA-seq"}}]},{"key":"targets","label":{"ja":"測定対象","en":"Target"},"type":"text","text":{"ja":null,"en":null}},{"key":"fragmentation","label":{"ja":"断片化","en":"Fragmentation"},"type":"text","text":{"ja":"GeneWiz社のプロトコルに準拠","en":"GeneWiz inc.'s protocol"}},{"key":"platform","label":{"ja":"プラットフォーム","en":"Platform"},"type":"vocabulary","terms":[{"code":"illumina-novaseq-6000","label":{"en":"Illumina NovaSeq 6000"}}]},{"key":"read-type","label":{"ja":"リードタイプ","en":"Read type"},"type":"vocabulary","terms":[{"code":"paired-end","label":{"ja":"ペアエンド","en":"Paired-end"}}]},{"key":"read-length","label":{"ja":"リード長","en":"Read length"},"type":"number","numbers":[{"value":150,"unit":"bp","high":null}]},{"key":"policies","label":{"ja":"利用ポリシー","en":"Data use policy"},"type":"vocabulary","terms":[{"code":"nbdc-data-sharing-policy-jgap000001","label":{"ja":"NBDC データ共有ポリシー (JGAP000001)","en":"NBDC data sharing policy (JGAP000001)"}}]}]}],"dataVolume":45555628382,"fileFormats":[{"code":"fastq","label":{"en":"FASTQ"}}]}