{"id":"JGAD000142","research":"hum0129","url":"https://humandbs.dbcls.jp/dataset/JGAD000142","datePublished":"2020-09-28","dateModified":"2020-11-18","values":[{"key":"access-criteria","label":{"ja":"アクセス制限","en":"Access criteria"},"type":"vocabulary","terms":[{"code":"controlled-access-type-1","label":{"ja":"制限公開（Type I）","en":"Controlled-access (Type I)"}}]},{"key":"type-of-data","label":{"ja":"データの種類","en":"Type of data"},"type":"text","text":{"ja":"NGS（ChIP-seq）","en":"NGS (ChIP-seq)"}}],"experiments":[{"label":"ChIP-seq","values":[{"key":"materials-and-participants","label":{"ja":"材料と対象者","en":"Materials and participants"},"type":"text","text":{"ja":"CHARGE症候群（ICD10：Q878）：2症例（2検体ずつ計4検体）","en":"CHARGE syndrome patient (ICD10: Q878): 3 cases\n(2 samples each, total 4 samples)"}},{"key":"disease","label":{"ja":"疾患","en":"Disease"},"type":"disease","diseases":[{"terms":[{"code":"Q878","label":{"ja":"その他の明示された先天奇形症候群，他に分類されないもの","en":"Other specified congenital malformation syndromes, not elsewhere classified"}}],"name":{"ja":"CHARGE症候群","en":"CHARGE syndrome patient"}}]},{"key":"health-status","label":{"ja":"健康状態","en":"Health status"},"type":"vocabulary","terms":[{"code":"affected","label":{"ja":"罹患","en":"Affected"}}]},{"key":"subject-count","label":{"ja":"対象者数","en":"Subject count"},"type":"number","numbers":[{"value":2,"unit":null,"high":null}]},{"key":"subject-count-type","label":{"ja":"対象者数の数え方","en":"Counted as"},"type":"vocabulary","terms":[{"code":"individual","label":{"ja":"人数","en":"Individual"}}]},{"key":"sample-description","label":{"ja":"試料説明","en":"Sample description"},"type":"text","text":{"ja":"CHARGE症候群患者由来皮膚線維芽細胞より樹立したiPS細胞から分化誘導した神経上皮様細胞から抽出したDNA\n抗ヒストン抗体（H3K27ac）を用いて免疫沈降","en":"DNAs extracted from long-term self-renewing neuroepithelial-like stem cells induced from iPS cells established from skin fibroblasts of CHARGE syndrome patients\nanti-histone antibody (H3K27ac) was used for immunoprecipitation"}},{"key":"tissue","label":{"ja":"組織","en":"Tissue"},"type":"vocabulary","terms":[{"code":"neuroepithelial-cell","label":{"en":"Neuroepithelial cell"}},{"code":"dermal-fibroblast","label":{"en":"Dermal fibroblast"}}]},{"key":"is-tumor","label":{"ja":"腫瘍 / 非腫瘍","en":"Tumor / normal"},"type":"vocabulary","terms":[{"code":"normal","label":{"ja":"非腫瘍","en":"Normal"}}]},{"key":"cell-line","label":{"ja":"細胞株","en":"Cell line"},"type":"vocabulary","terms":[{"code":"ipsc","label":{"en":"iPSC"}}]},{"key":"sample-provider","label":{"ja":"試料の入手元","en":"Sample provider"},"type":"text","text":{"ja":null,"en":null}},{"key":"experimental-method","label":{"ja":"実験方法","en":"Experimental method"},"type":"vocabulary","terms":[{"code":"chip-seq","label":{"en":"ChIP-seq"}}]},{"key":"targets","label":{"ja":"測定対象","en":"Target"},"type":"text","text":{"ja":null,"en":null}},{"key":"reagents","label":{"ja":"試薬キット","en":"Reagent kit"},"type":"vocabulary","terms":[{"code":"kapa-hyper-prep-kit","label":{"en":"KAPA Hyper Prep Kit"}}]},{"key":"fragmentation","label":{"ja":"断片化","en":"Fragmentation"},"type":"text","text":{"ja":"超音波断片化（Covaris S2）","en":"Ultrasonic fragmentation (Covaris S2)"}},{"key":"platform","label":{"ja":"プラットフォーム","en":"Platform"},"type":"vocabulary","terms":[{"code":"illumina-hiseq-2500","label":{"en":"Illumina HiSeq 2500"}}]},{"key":"read-type","label":{"ja":"リードタイプ","en":"Read type"},"type":"vocabulary","terms":[{"code":"single-end","label":{"ja":"シングルエンド","en":"Single-end"}}]},{"key":"read-length","label":{"ja":"リード長","en":"Read length"},"type":"number","numbers":[{"value":50,"unit":"bp","high":null}]},{"key":"reference-sequence","label":{"ja":"参照ゲノム","en":"Reference genome"},"type":"vocabulary","terms":[{"code":"grch37","label":{"en":"GRCh37"}}]},{"key":"has-phenotype-data","label":{"ja":"表現型データの有無","en":"Phenotype data"},"type":"vocabulary","terms":[{"code":"included","label":{"ja":"あり","en":"Included"}}]},{"key":"policies","label":{"ja":"利用ポリシー","en":"Data use policy"},"type":"vocabulary","terms":[{"code":"nbdc-data-sharing-policy-jgap000001","label":{"ja":"NBDC データ共有ポリシー (JGAP000001)","en":"NBDC data sharing policy (JGAP000001)"}}]}]}],"dataVolume":10721973424,"fileFormats":[{"code":"fastq","label":{"en":"FASTQ"}},{"code":"bigwig","label":{"en":"bigWig"}}]}