{"id":"JGAD000089","research":"hum0065","url":"https://humandbs.dbcls.jp/dataset/JGAD000089","datePublished":"2020-09-28","dateModified":"2020-11-18","values":[{"key":"access-criteria","label":{"ja":"アクセス制限","en":"Access criteria"},"type":"vocabulary","terms":[{"code":"controlled-access-type-1","label":{"ja":"制限公開（Type I）","en":"Controlled-access (Type I)"}}]},{"key":"type-of-data","label":{"ja":"データの種類","en":"Type of data"},"type":"text","text":{"ja":"NGS（Exome）","en":"NGS (Exome)"}}],"experiments":[{"label":"WES","values":[{"key":"materials-and-participants","label":{"ja":"材料と対象者","en":"Materials and participants"},"type":"text","text":{"ja":"大腸がん：8症例、肺がん：2症例、子宮頸部小細胞がん：2症例\n1. 血球細胞：12検体（大腸がん 8検体、肺がん 2検体、子宮頸部小細胞がん 2検体）\n2. CTOS（初代）：患者さんの腫瘍組織から調整したCTOS：5検体（大腸がん2検体、肺がん2検体、子宮頸部小細胞がん1検体）\n3. CTOS（継代）：\na. 2. のCTOS をマウスに移植して得られた腫瘍組織から調整したCTOS：14検体\n1代：1検体（大腸がん1検体）\n2代：4検体（子宮頸部小細胞がん1検体、大腸がん3検体）\n3代：5検体（大腸がん3検体、肺がん2検体）\n5代：4検体（大腸がん2検体、肺がん2検体）\nb. 2. のCTOSをin vitroで培養した細胞から調整したCTOS：3検体（肺がん2検体、大腸がん1検体）\n2代：1検体（肺がん1検体）\n5代：2検体（肺がん1検体、大腸がん1検体）","en":"colorectal cancer: 8 cases, lung cancer: 2 cases, small cell carcinoma of uterine cervix: 2case\n1. blood cells: 12 samples\n(colorectal cancer: 8 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 2samples)\n2. CTOS (primary culture): CTOS derived from patient tumors: 5 samples\n(colorectal cancer: 2 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 1 sample)\n3. CTOS (subculture):\na. CTOS prepared from a CTOS (the same as 2)-derived mouse xenograft: 14 samples\n1st subculture: 1 sample (colorectal cancer: 1 sample)\n2nd subculture: 4 samples (small cell carcinoma of uterine cervix: 1 sample, colorectal cancer: 3 samples)\n3rd subculture: 5 samples (colorectal cancer: 3 samples, lung cancer: 2 samples)\n5th subculture: 4 samples (colorectal cancer: 2 samples, lung cancer: 2 samples)\nb. CTOS (the same as 2) cultured in vitro: 3 samples (lung cancer: 2 samples, colorectal cancer: 1 sample)\n2nd subculture: 1 sample (lung cancer: 1 sample)\n5th subculture: 2 samples (lung cancer: 1 sample, colorectal cancer: 1 sample)"}},{"key":"health-status","label":{"ja":"健康状態","en":"Health status"},"type":"vocabulary","terms":[{"code":"affected","label":{"ja":"罹患","en":"Affected"}}]},{"key":"subject-count","label":{"ja":"対象者数","en":"Subject count"},"type":"number","numbers":[{"value":12,"unit":null,"high":null}]},{"key":"subject-count-type","label":{"ja":"対象者数の数え方","en":"Counted as"},"type":"vocabulary","terms":[{"code":"individual","label":{"ja":"人数","en":"Individual"}}]},{"key":"sample-description","label":{"ja":"試料説明","en":"Sample description"},"type":"text","text":{"ja":"血球細胞およびCTOSから抽出したDNA","en":"DNAs extracted from blood cells and CTOSs"}},{"key":"tissue","label":{"ja":"組織","en":"Tissue"},"type":"vocabulary","terms":[{"code":"peripheral-blood","label":{"ja":"末梢血","en":"Peripheral blood"}},{"code":"ctos","label":{"en":"CTOS"}}]},{"key":"is-tumor","label":{"ja":"腫瘍 / 非腫瘍","en":"Tumor / normal"},"type":"vocabulary","terms":[{"code":"mixed","label":{"ja":"混在","en":"Mixed"}}]},{"key":"sample-provider","label":{"ja":"試料の入手元","en":"Sample provider"},"type":"text","text":{"ja":null,"en":null}},{"key":"experimental-method","label":{"ja":"実験方法","en":"Experimental method"},"type":"vocabulary","terms":[{"code":"wes","label":{"en":"WES"}}]},{"key":"targets","label":{"ja":"測定対象","en":"Target"},"type":"text","text":{"ja":null,"en":null}},{"key":"reagents","label":{"ja":"試薬キット","en":"Reagent kit"},"type":"vocabulary","terms":[{"code":"sureselect-human-all-exon-v5-lincrna","label":{"en":"SureSelect Human All Exon V5 + lincRNA"}}]},{"key":"fragmentation","label":{"ja":"断片化","en":"Fragmentation"},"type":"text","text":{"ja":"超音波断片化（Covaris E220）","en":"Ultrasonic fragmentation (Covaris, E220)"}},{"key":"platform","label":{"ja":"プラットフォーム","en":"Platform"},"type":"vocabulary","terms":[{"code":"illumina-hiseq-2000","label":{"en":"Illumina HiSeq 2000"}}]},{"key":"read-type","label":{"ja":"リードタイプ","en":"Read type"},"type":"vocabulary","terms":[{"code":"paired-end","label":{"ja":"ペアエンド","en":"Paired-end"}}]},{"key":"read-length","label":{"ja":"リード長","en":"Read length"},"type":"number","numbers":[{"value":100,"unit":"bp","high":null}]},{"key":"has-phenotype-data","label":{"ja":"表現型データの有無","en":"Phenotype data"},"type":"vocabulary","terms":[{"code":"included","label":{"ja":"あり","en":"Included"}}]},{"key":"policies","label":{"ja":"利用ポリシー","en":"Data use policy"},"type":"vocabulary","terms":[{"code":"nbdc-data-sharing-policy-jgap000001","label":{"ja":"NBDC データ共有ポリシー (JGAP000001)","en":"NBDC data sharing policy (JGAP000001)"}}]}]}],"dataVolume":1174271264096,"fileFormats":[{"code":"fastq","label":{"en":"FASTQ"}}]}