{"id":"JGAD000065","research":"hum0068","url":"https://humandbs.dbcls.jp/dataset/JGAD000065","datePublished":"2020-09-28","dateModified":"2024-10-17","values":[{"key":"access-criteria","label":{"ja":"アクセス制限","en":"Access criteria"},"type":"vocabulary","terms":[{"code":"controlled-access-type-1","label":{"ja":"制限公開（Type I）","en":"Controlled-access (Type I)"}}]},{"key":"type-of-data","label":{"ja":"データの種類","en":"Type of data"},"type":"text","text":{"ja":"NGS（PCR amplicon Seq）","en":"NGS (PCR amplicon Seq)"}}],"experiments":[{"label":"RNA Amplicon-seq","values":[{"key":"materials-and-participants","label":{"ja":"材料と対象者","en":"Materials and participants"},"type":"text","text":{"ja":"肺腺がん（ICD10：C349）：8症例（7検体）","en":"lung adenocarcinoma (ICD10: C349): 8 cases (7 samples)"}},{"key":"disease","label":{"ja":"疾患","en":"Disease"},"type":"disease","diseases":[{"terms":[{"code":"C349","label":{"ja":"気管支又は肺，部位不明","en":"Malignant neoplasm: Bronchus or lung, unspecified"}}],"name":{"ja":"肺腺がん","en":"lung adenocarcinoma"}}]},{"key":"health-status","label":{"ja":"健康状態","en":"Health status"},"type":"vocabulary","terms":[{"code":"affected","label":{"ja":"罹患","en":"Affected"}}]},{"key":"subject-count","label":{"ja":"対象者数","en":"Subject count"},"type":"number","numbers":[{"value":8,"unit":null,"high":null}]},{"key":"subject-count-type","label":{"ja":"対象者数の数え方","en":"Counted as"},"type":"vocabulary","terms":[{"code":"individual","label":{"ja":"人数","en":"Individual"}}]},{"key":"sample-description","label":{"ja":"試料説明","en":"Sample description"},"type":"text","text":{"ja":"肺腺がん組織より抽出したRNAを逆転写したcDNA","en":"cDNA were synthesized from mRNA extracted from lung adenocarcinomas"}},{"key":"tissue","label":{"ja":"組織","en":"Tissue"},"type":"vocabulary","terms":[{"code":"lung","label":{"ja":"肺","en":"Lung"}}]},{"key":"is-tumor","label":{"ja":"腫瘍 / 非腫瘍","en":"Tumor / normal"},"type":"vocabulary","terms":[{"code":"tumor","label":{"ja":"腫瘍","en":"Tumor"}}]},{"key":"sample-provider","label":{"ja":"試料の入手元","en":"Sample provider"},"type":"text","text":{"ja":null,"en":null}},{"key":"experimental-method","label":{"ja":"実験方法","en":"Experimental method"},"type":"vocabulary","terms":[{"code":"amplicon-seq","label":{"en":"Amplicon-seq"}}]},{"key":"targets","label":{"ja":"測定対象","en":"Target"},"type":"text","text":{"ja":"EGFR・KRAS領域、および、EML4-ALK・KIF5B-RET領域\n検体1：EML4-ALK保有1症例（EML4-ALK領域）と KIF5B-RET保有1症例（KIF5B-RET領域）の増幅産物の混合検体（1検体）\n検体2～7：EGFRとKRAS領域の増幅産物（6検体）","en":"sample 1: Amplicons from EML4-ALK positive case (EML4-ALK region) and KIF5B-RET positive case (KIF5B-RET region) were mixed (1 sample)\nsample 2 - 7: Amplicons from 6 cases (EGFR and KRAS regions)"}},{"key":"reagents","label":{"ja":"試薬キット","en":"Reagent kit"},"type":"vocabulary","terms":[{"code":"nanopore-sequencing-kit","label":{"en":"Nanopore Sequencing Kit"}}]},{"key":"fragmentation","label":{"ja":"断片化","en":"Fragmentation"},"type":"text","text":{"ja":"PCR","en":"PCR"}},{"key":"platform","label":{"ja":"プラットフォーム","en":"Platform"},"type":"vocabulary","terms":[{"code":"oxford-nanopore-technologies-minion","label":{"en":"Oxford Nanopore Technologies MinION"}}]},{"key":"read-type","label":{"ja":"リードタイプ","en":"Read type"},"type":"vocabulary","terms":[{"code":"single-end","label":{"ja":"シングルエンド","en":"Single-end"}}]},{"key":"read-length","label":{"ja":"リード長","en":"Read length"},"type":"number","numbers":null},{"key":"policies","label":{"ja":"利用ポリシー","en":"Data use policy"},"type":"vocabulary","terms":[{"code":"nbdc-data-sharing-policy-jgap000001","label":{"ja":"NBDC データ共有ポリシー (JGAP000001)","en":"NBDC data sharing policy (JGAP000001)"}}]}]}],"dataVolume":932648048,"fileFormats":[{"code":"fastq","label":{"en":"FASTQ"}}]}